Responding to the call of American Muslim organizations, worshippers laid out their prayer mats at a park in front of the president's residence.
Wearing traditional Palestinian keffiyeh scarves or the colors of the Palestinian flag, protesters also held placards denouncing Israeli occupation of Quds and Palestine.
On Wednesday, Trump declared that the US recognizes Quds as the capital of the Israeli regime and announced the relocation of the US embassy from Tel Aviv to Quds - turning his back on decades of American and international diplomacy.
"Trump does not own a piece of soil of Jerusalem and Palestine. He owns the Trump Tower. He can give it away to the Israelis," Nihad Awad, executive director of the Council on American-Islamic Relations (CAIR), told AFP.
The president is "empowering Christian religious extremism in the US," he added.
Source: World News