In a message on Saturday, the Leader said the announcement by the Armed Forces that a human error caused the crash made the grief for those killed in the incident even heavier.
Ayatollah Khamenei once again expressed deep condolences to the families of the victims and prayed that God would grant them fortitude and calm.
The Leader also called on the General Staff of the Armed Forces to investigate the possible failures and faults and take all necessary measures to make sure such incidents will not happen again.
In a statement Saturday, the general staff of the armed forces touched on the tense situation in the hours after Iran's missile attack on US bases in Iraq, saying Tehran was on the highest level of alert "in response to threats by the criminal American president and military commanders" to hit a large number of Iranian targets.
“In the hours after the missile strikes, flights by the warplanes of the terrorist American forces around the country increased, and some reports of aerial targets heading toward strategic centers of the country were received by defense units while a number of targets appeared on some radar screens which raised the sensitivity of the air defense networks.
“Under such sensitive and critical conditions, the Ukrainian Airlines flight 752 took off from the Imam Khomeini airport and while rotating, it was placed completely in the position of approaching a sensitive military center in the altitude and trajectory of an enemy target.
“In these circumstances, the plane was unintentionally hit due to human error, which unfortunately resulted in the martyrdom of a number of our dear compatriots and the death of a number of foreign nationals,” it said.
All 176 people on the Ukraine International Airlines (UIA) flight died in the crash this week just after take-off from Tehran on a flight to Kiev.
“The Islamic Republic of Iran deeply regrets this disastrous mistake,” President Hassan Rouhani wrote on Twitter. "My thoughts and prayers go to all the mourning families. I offer my sincerest condolences.”